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Our new kit for ASFV detection with 2 IPCs!

Our new kit for ASFV detection with 2 IPCs!

Discover our new version of the kit for ASFV detection, a kit with 2 IPCs!

The Bio-T kit® ASFV Universal contains a ready-to-use Master Mix allowing the detection, in the same reaction well, of:

- African Swine Fever Virus (ASFV) thanks to a 6-FAM channel,

- An endogenous internal positive control IPC (GAPDH) to assess the presence of sufficient amount of host cells, sample integrity, nucleic acids extraction quality, and absence of RT-PCR inhibitors, thanks to a Cy5 channel,

- An exogenous internal positive control IC (synthetic DNA) to ensure the quality of the extraction, thanks to a VIC channel. 

SEE THE PRODUCT SHEET

THE + OF THIS KIT

- A ready-to-use Master Mix

- 2 IPCs ensure the high-quality results on all types of matrices samples

- PCR run time: about 1h

- This kit was developed according to the French regulatory standard NF U47-600-2 edited by AFNOR, and validated by the INIA laboratory

 

SAMPLE TYPES:

Individual analysis or by pool up to 10 or 20 according to the matrix & extraction method:

SAMPLE TYPE

LOD METHOD

POOL

Whole blood (EDTA), serum, cell culture supernatant

2.5 x 102.8 HAU/ ml

Up to 20

Organs (spleens, tonsils, lymph nodes)

1 x 102.8 HAU/ ml

Up to 10

Swabs (blood or exudates)

1 x 103.8 HAU/ ml

Individual


EXTRACTION – METHOD VALIDATED ON:

- Magnetic beads extraction (e.g.: Biosellal – BioExtract® SuperBall® Cat. N° BES384) classical program 38 min and short program 19 min according to the matrix

- Spin column (e.g.: Roche – High Pure PCR Template Preparation kit)


RT-PCR DEVICES

This kit is compatible with ABI PRISM® 7500 Fast, QuantStudio5®, Aria MxTM, CFX96, and all thermal cyclers with at least 6-FAM, VIC (HEX), and Cy5 channels.


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