Digital PCR: For accurate and reliable paratuberculosis diagnosis

Posted On: 01/27/2025

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Driven by a spirit of innovation, we have developed a new diagnostic tool for Paratuberculosis, enabling the detection and quantification of Mycobacterium avium paratuberculosis by digital PCR (dPCR): the Bio-dPCR kit™ Mycobacterium avium paratuberculosis AQ. This triplex dPCR kit enables digital PCR to detect and quantify Mycobacterium avium paratuberculosis (MAP) IS900  multicopy and F57 monocopy genes. It also contains an exogenous internal control (IPC ) to guarantee reliable results, even on the most complex matrices.

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✔ Developed specifically on ThermoFisher's QuantStudio™ Absolute Q™ device, you can contact our technical support for use on another device
✔ Detection and quantification quality ensured by the two target genes: IS900 and F57
✔ Most accurate technology on the market with no standard range
✔ Master Mix ready to use
✔ Contains the plastic consumables needed to launch the PCR digitizer
✔ Can be used on individual and environmental feces, pool of feces, cloths, and boot cloths

Discover all our solutions for optimal paratuberculosis diagnosis

The Bio-dPCR kit™ Mycobacterium avium paratuberculosis AQ comes with a full range of tools to meet your paratuberculosis diagnostic needs:

  • Bio-T kit® MAP IS900 & F57 triplex (ref. BIOTK162 - under development): A triplex PCR kit for the detection and relative and absolute quantification of Mycobacterium avium paratuberculosis (MAP) IS900 multicopy and F57 monocopy genes by real-time PCR.
  • A MAP pretreatment kit for kits (ref. BIOPREPMAP): Bio-T kit® Mycobacterium avium paratuberculosis, Bio-T kit® MAP IS900 & F57 triplex, and the Bio-dPCR kit™ Mycobacterium avium paratuberculosis AQ.

  • BioLisa® kit MAP Ab V2 (ref. BIOLK020): an indirect ELISA kit for the detection of antibodies to Mycobacterium avium paratuberculosis in individual sera from cattle, sheep, and goats.

How can you use our solutions to address your field challenges? 

✔ Determination of the animal status on an individual fecal sample by relative quantification compared to the Mycobacterium avium paratuberculosis quantitative reference standard (Labocea x BioSellal) using our real-time PCR kits : Bio-T kit® Mycobacterium avium paratuberculosis and Bio-T kit® MAP IS900 & F57 Triplex
✔ Longitudinal monitoring of bacterial load within the herd on various matrices through absolute quantification of IS900 and F57 genes with Bio-T kit® MAP IS900 & F57 Triplex, and IS900 gene with Bio-T kit® Mycobacterium avium paratuberculosis
✔ Longitudinal monitoring of bacterial load within the herd on environmental samples (environmental feces, cloths, and boot cloths, and pediatric swabs) through absolute quantification, without the need to perform a standard curve, using our Bio-dPCR kit™ Mycobacterium avium paratuberculosis AQ

Your trusted partner for paratuberculosis diagnosis

With the launch of the Bio-dPCR kit™ Mycobacterium avium paratuberculosis AQ, we now have a complete range of tools for paratuberculosis diagnostics, enabling us to respond optimally to your needs.

To learn more about our tools or discuss your diagnostic projects, please contact us today.

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