An innovation that redefines quantitative analysis
Digital PCR (Digital Polymerase Chain Reaction) represents a major advance in the field of molecular diagnostics, enabling more precise, reproducible and reliable analyses than traditional PCR techniques.
How does digital PCR work?
Unlike conventional PCR, which amplifies a sample as a whole, digital PCR divides the sample into thousands of independent reactions, each with a probability of containing zero or one target molecule. Each reaction acts as an individual PCR test. After amplification, the presence or absence of the target is determined in each reaction. By combining the results of all these micro-reactions, and through the use of the statistical fish law, it is possible to precisely quantify the amount of the target present in the initial sample.
Digital PCR works in three key stages:
1. Sample partitioning :
The sample is divided into a large number of microreactions, each distributed in individual compartments.
2. Amplification :
Each compartment undergoes PCR amplification.
3. Analysis :
The number of compartments containing the target is counted, enabling quantification with unprecedented precision.
The benefits of digital PCR
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MAXIMUM PRECISION
Digital PCR offers unprecedented precision, enabling reproducible quantification of small quantities of nucleic acid.
ABSOLUTE QUANTIFICATION
Unlike traditional quantitative PCR, which relies on calibration curves, digital PCR provides absolute quantification without the need for standardization.
RELIABLE RESULTS
This technology reduces errors due to sample variability, offering more reliable results, even with complex or heterogeneous samples.
Digital PCR applications
Digital PCR opens up a wide range of application possibilities, particularly in fields where precision and absolute quantification are crucial:
➜ Diagnosis of infections: Early detection and quantification of pathogens as in the case of viral or bacterial infections.
➜ Oncology: Detection of specific genetic mutations, analysis of tumor biomarkers, and monitoring of relapse in the follow-up of cancer patients.
➜ Genetics: Quantification of rare genetic variations or point mutations, useful for human and animal genetics research.
➜ Environmental biology: monitoring pathogens in the environment, such as detecting DNA from invasive species or pathogens in wastewater or soil.
➜ Clinical research: studies on gene expression, biomarkers and the identification of new therapeutic targets.
Why choose digital PCR?
For research laboratories, diagnostic clinics and biotech companies, digital PCR is a real asset. Its ability to deliver accurate, reproducible results even with complex samples makes it the preferred choice for the most demanding applications.
By adopting digital PCR, you benefit from the reliability and precision you need to make more informed decisions, whether in clinical diagnostics, basic research or industrial applications.
BIOSELLAL application
BioSellal is now using this technique to diagnose paratuberculosis, bringing unprecedented precision to the absolute quantification of Mycobacterium avium paratuberculosis. The technique is also available for the quantification of reference materials and standard samples.