Babesiosis and Theileriosis PCR Kit
Bio-T kit® Babesia spp & Theileria spp
The Babesiosis and Theileriosis PCR kit is a ready-to-use kit for the relative quantification and detection of Babesia and Theileria by real-time PCR, for the diagnosis of Babesiosis and Theileriosis in ruminants. This Babesiosis and Theileriosis PCR test contains an endogenous IPC internal positive control.
✔ Babesiosis and Theileriosis duplex PCR kit developed in compliance with French standard U47-600-2 (AFNOR)
✔ Rapid thawing of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Reliable detection of Babesia spp and Theileria spp by real-time PCR thanks to endogenous IPC to ensure high-quality results on all matrices, even the most difficult ones
✔ Matrices: Whole blood (on EDTA tubes). Individual analyses.
✔ Kit developed on AriaMx™ (Agilent Technologies, Fast ramping by default). It is compatible with all thermal cyclers with at least 6-FAM and Cy5 reading channels.
✔ Extraction and amplification program common to all kits for Ruminant species
✔ French manufacturing
- Method
- qPCR
- Species
- Ruminants
- Pathogen
- Babesia spp
Theileria spp - Brand
- Biosellal
Resources
Download our marketing documents for more information.
About Babesiosis
Babesiosis is an infectious disease caused by protozoa of the Babesia genus, mainly Babesia bovis and Babesia bigemina in cattle, and Babesia ovis in sheep. The disease is transmitted by vector ticks of the genus Rhipicephalus, notably Rhipicephalus (Boophilus) microplus. Babesia parasites in ruminants are found inside erythrocytes, also known as red blood cells. These parasites belong to a group of hematophagous protozoa, and once they infect an animal, they multiply inside the red blood cells, causing destruction of these blood cells. This can lead to hemolytic anemia in infected animals.
Symptoms of Babesiosis vary according to the Babesia species involved, but can include fever, anorexia, jaundice, hemolytic anemia, respiratory disorders and reduced milk production in cattle. In sheep, symptoms can include anemia, weight loss, weakness, and sometimes sudden death. Early diagnosis of Babesiosis is essential for rapid intervention and appropriate control measures. Babesiosis PCR kits such as the one developed by BioSellal offer an accurate, rapid and reliable solution for the detection of Babesia spp in animal samples, enabling farmers and veterinarians to intervene rapidly to safeguard the health of their herds.
About Theileriosis
Theileriosis is an infectious parasitic disease caused by protozoa of the Theileria genus, mainly Theileria annulata and Theileria parva in cattle. These parasites are transmitted by ticks of the genus Rhipicephalus. Theileria parasites in ruminants are also present inside erythrocytes, as are Babesia parasites. Once they infect an animal, Theileria parasites multiply inside red blood cells, leading to cell cycle disruption and red blood cell degradation. This infection can cause a disease called theileriosis, characterized by anemia and other clinical symptoms in infected ruminants.
Symptoms of Theileriosis vary according to the species of Theileria involved, but can include fever, anemia, decreased appetite, weight loss, lethargy and respiratory signs in cattle. The disease can cause significant morbidity and mortality in regions where it is endemic. Early diagnosis of Theileriosis is essential for rapid intervention and the implementation of appropriate control measures. Theileriosis PCR kits such as the one developed by BioSellal offer an accurate, rapid and reliable solution for the detection of Theileria spp in animal samples, enabling farmers and veterinarians to intervene rapidly to safeguard the health of their herds.
Made in France
Opt for quality and local know-how.
Iso-certified
A guarantee of quality in line with international standards.
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.