Bovine Viral Diarrhea PCR Kit
Bio-T kit® BVDV/BDV Universal
The Bovine Viral Diarrhea PCR kit is a ready-to-use kit for the detection of BVDV / BDV (ruminant pestiviruses) by real-time PCR for the diagnosis of viral diarrhea in cattle. This BVDV / BDV test contains an exogenous IC internal positive control and an endogenous IPC internal positive control. It has been validated by the French BVD National Reference Laboratory (ANSES, Ploufragan-Plouzané-Niort) for dry ear biopsies in mixtures of 25 and for serum.
✔ Triplex developed in compliance with French standard U47-600-2 (AFNOR) and validated by the French BVD NRL (ANSES, Ploufragan-Plouzané-Niort)
✔ Rapid defrosting of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Reliable detection of BVDV by real-time PCR thanks to 2 IPCs: an exogenous IPC and an endogenous IPC to ensure high-quality results on all matrices, even the most difficult ones
✔ Matrices: whole blood (on EDTA tubes), serum*, dry, wet (BAH) and liquid (BAL) ear biopsy*, milk, organ (fetal spleen, injured lung, rectum or abomasum). Individual analyses or mixtures of up to 10 or 25, depending on the matrix**
✔ Opt for the most cost-effective solution for diagnosing Bovine Viral Diarrhea on individual ear biopsies and in mixtures of up to 25 by using this kit in combination with - The ILV rapid lysis buffer (5 min), validated by the LNR BVD (ANSES, Ploufragan-Plouzané-Niort) - The Myra liquid handling system to prepare mixtures of up to 25 ear biopsies
✔ Kit developed on ABI PRISM® 7500 Fast (Applied Biosystems) and AriaMx™ (Agilent Technologies, ramping Fast by default). The CFX96™ (Bio-Rad), LightCycler® 480 (Roche), and Rotor-Gene Q (Qiagen) have also been validated. It is compatible with all thermal cyclers with at least reading channels 6-FAM, VIC, and Cy5.
✔ Kit validated on Mic qPCR cycler (Bio molecular systems) with 60 min run
✔ Made in France
* Only the ear biopsy and serum matrices have been evaluated by the LNR BVD (ANSES, Ploufragan-Plouzané-Niort) as part of the non-IPI guarantee.
** Please refer to the current non-IPI guarantee recommendations for mix size and age of animals according to matrices.
- Method
- qRT-PCR
- Species
- Ruminants
- Pathogen
- Bovine Viral Diarrhea Virus
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
About
Bovine Viral Diarrhea
Caused by the Bovine Viral Diarrhea Virus (BVDV), a member of the pestivirus family, Bovine Viral Diarrhea (BVD) is a contagious infection that mainly affects cattle.
Symptoms of BVD vary considerably, ranging from mild subclinical forms to severe acute infections. Common signs include fever, decreased appetite, depression, nasal discharge, diarrhea, and in severe cases, oral and gastrointestinal lesions. In pregnant females, BVDV infection can lead to abortions, congenital malformations, or the birth of persistently infected immunotolerant (PI) calves, true vectors of the virus.
Diagnosis & prevention of Bovine Viral Diarrhea
Bovine Viral Diarrhea can have devastating consequences for the health and well-being of cattle. Early diagnosis of BVD is therefore crucial to effective disease management.
Bovine Viral Diarrhea PCR kits, such as the Bio-T kit® BVDV/BDV Universal, play an essential role in the rapid and accurate detection of BVD. The matrices used are ear biopsies for individual analysis and blending up to 25 samples, blood for individual analysis and blending up to 20 samples, serum for individual analysis and blending up to 20 samples. Prevention relies on vaccination, herd management and biosecurity strategies to limit the spread of the virus.
Made in France
Opt for quality and local know-how.
Iso-certified
A guarantee of quality in line with international standards.
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.