Paratuberculosis ELISA kit

BioLisa® kit MAP Ab V2

BIOLK020

Number of reactions per kit

Description

The Paratuberculosis ELISA kit is an indirect single-step ELISA test for the detection of antibodies to Mycobacterium avium paratuberculosis (MAP) in individual serum samples from ruminants (cattle, sheep, goats). This ELISA test is used to diagnose paratuberculosis in ruminants.

Paratuberculosis ELISA kit developed following French standards U47-310 and U47-019 (AFNOR)

Ready-to-use and colored reagents for fast, easy discrimination

Sensitive, specific MAP indirect ELISA test for detecting exposure of bovines, ovines and caprines to Mycobacterium avium paratuberculosis

Short incubation protocol

To improve the monitoring of your results over time, we offer a ready-to-use low-positive sample (MRI, Internal Reference Material, BioLisa® MRI Serum MAP (individual), ref. ESTMAPB001). This sample allows you to ensure the reliability of the test using a control card. We recommend using it for each test.

Matrices: Individual bovine, ovine, or caprine serum sample

Made in France

Method
Indirect ELISA

Species
Ruminants

Pathogen
Mycobacterium avium paratuberculosis

Brand
Biosellal

Resources

Download our marketing documents below for more information.

About Paratuberculosis (MAP)

Bovine paratuberculosis, also known as Johne's disease, is a chronic bacterial infection mainly affecting cattle. The disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), a bacterium that infects the intestinal tract of animals. Symptoms of bovine paratuberculosis include chronic diarrhoea, weight loss, reduced milk production, and increased mortality in infected animals.

Diagnosis & prevention of paratuberculosis (MAP)

This disease can lead to significant economic losses for livestock farmers due to its effects on animal health and productivity. Early diagnosis of Bovine Paratuberculosis is essential to implement effective control measures and limit its spread within the herd. Paratuberculosis ELISA kits such as the one developed by BioSellal are widely used for the detection of antibodies to Mycobacterium avium paratuberculosis (MAP). These ELISAs offer a rapid and accurate method of identifying infected animals, enabling farmers to make informed decisions to protect the health of their herds.

Made in France

Opt for quality and local know-how.

Iso-certified

A guarantee of quality in line with international standards.

Secure payment

Your transactions are completely secure.

Availability & support

Our technical support team is on hand to help you with your analyses.

Frequently asked questions

Each real-time PCR kit includes :

  • Ready-to-use Master Mix (blue color)
  • Positive amplification control, containing targeted pathogens - EPC
  • Negative amplification control - water.
  • Exogenous IPC (Internal positive control)

Each ELISA kit includes at least :

  • Test plate with 12 x 8-well strips
  • Sample diluent
  • Negative control
  • Positive control
  • 100X or ready-to-use conjugate
  • Conjugate diluent
  • Wash solution
  • Substrate
  • Stop solution
  • Plate adhesives

Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.

To ensure the integrity and efficiency of your kits, follow these storage recommendations:

Real-time PCR kits:

On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.

ELISA kits:

On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.

By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.

  • The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.

  • The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.

  • The shelf-life of an ELISA kit is 15 or 18 months.

At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.

Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.

The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.

All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.

Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.

At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.

Our various departments are always ready to answer your questions and help you. Here's how to contact us:

Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)

  • Mail: contact@biosellal.com
  • Phone: +33 (0)4 26 78 32 48

Sales Department (for all customer inquiries)

  • Mail: commercial@biosellal.com
  • Phone: +33 (0)4 26 78 32 57

Technical Support (for technical assistance)

  • Mail: tech@biosellal.com
  • Tel: +33 (0)4 26 78 32 48

Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!

Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.

All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.

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