Avian and swine influenza PCR kit
Bio-T kit® Avian & Swine Influenza Virus V2
The Avian and Swine Influenza PCR Kit is a ready-to-use kit for the detection of avian and swine Influenza virus type A (ASIV) genomes by real-time PCR, for the diagnosis of Avian Influenza and Swine Influenza. This Avian and Swine Influenza PCR test contains an endogenous IPC internal positive control and an exogenous IC internal control. It has been validated by the French National Reference Laboratory for Avian Influenza (ANSES, Ploufragan-Plouzané-Niort).
✔ Triplex Avian and Swine Influenza PCR kit developed following French standard U47-600-2 (AFNOR) and validated by the French Avian Influenza LNR (ANSES, Ploufragan-Plouzané-Niort)
✔ Rapid thawing of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Reliable detection of Avian and Swine Influenza by real-time PCR thanks to 2 IPCs: an exogenous IPC and an endogenous IPC to ensure high-quality results on all matrices, even the most difficult ones
✔ Detection of verified endogenous IPC on a wide range of species (domestic animals and wildlife)
✔ Sensitivity & specificity of Avian Influenza diagnosis with our extraction kits: 100% on organs or organ shreds (intestine, brain), swabs, and environmental samples such as wipes and pedichiffs.
✔ Matrices: nasal swab, organ or organ shred supernatant, tracheobronchial wash, allantoic fluid, or cell culture supernatant. Individual analyses or mixtures of up to 5 depending on the sample type and, unless otherwise specified, on the animal species, geographical origin, and date of sampling.
✔ Kit developed on the AriaMx™ (Agilent Technologies, Fast ramping by default) and confirmed on ABI PRISM® 7500 Fast (Applied Biosystems) in standard ramping, QuantStudio™ 5 (Applied Biosystems) in standard ramping, CFX96™ (Bio-Rad) in standard ramping, Mic qPCR Cycler (Bio Molecular Systems) in standard ramping and LightCycler® 480 (Roche Diagnostics) in standard ramping. It is compatible with all thermal cyclers with at least reading channels 6-FAM, VIC, and Cy5. For further information, don't hesitate to contact our technical support.
✔ Kit validated on Mic qPCR cycler (Bio molecular systems)
✔ Made in France
- Method
- qRT-PCR
- Species
- Avian
Swine - Pathogen
- Avian influenza virus
Porcine influenza virus - Brand
- Biosellal
Resources
Download our marketing documents below for more information.
About Avian Influenza
Avian influenza, also known as bird flu, is a viral disease that mainly affects birds, particularly poultry such as chickens, turkeys and ducks. It is caused by various subtypes of the influenza A virus, including the H5N1, H7N9 and H9N2 strains, among others.
➜ Transmission: Avian flu is transmitted mainly through direct contact with infected birds, as well as through respiratory secretions, feces and eye secretions of sick birds. Wild birds, particularly ducks and geese, often carry the virus and can spread it to domestic birds.
➜ Symptoms in birds: Clinical signs of avian flu in birds can vary, but typically include sudden loss of appetite, decreased egg production, difficulty breathing, diarrhea, nasal congestion, swelling of the face and neck, and neurological signs. In severe cases, the disease can rapidly prove fatal for birds.
➜ Human health risks: Although avian flu is primarily a disease of birds, some strains, such as H5N1 and H7N9, have demonstrated the ability to cause serious infections in humans. People in close contact with infected birds or their secretions, particularly poultry farm workers, can be exposed to the virus. Symptoms in humans can include fever, muscle aches, coughing, breathing difficulties and, in some cases, serious complications leading to death.
Diagnosis & prevention of Avian Influenza
DIAGNOSIS
Early diagnosis of Avian Influenza is essential. Avian Influenza PCR kits, like the one developed by BioSellal, offer a rapid and accurate solution for detecting the presence of Avian Influenza viruses in animal samples such as swabs, organs, as well as environmental matrices such as rags and pedichiffonnettes. Avian influenza remains a major concern for the poultry industry and public health due to its potential for rapid spread and its ability to cause serious illness in birds and, in some cases, humans. Ongoing surveillance, prevention and control efforts are essential to mitigate the risks associated with this disease.
PREVENTION
Measures to prevent and control avian influenza include quarantine of infected birds, safe disposal of sick animals, vaccination in certain cases, strict hygiene practices on poultry farms, epidemiological surveillance of viral strains, and restrictions on the trade and movement of live birds.
Made in France
Opt for quality and local know-how.
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A guarantee of quality in line with international standards.
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.