Digital Dermatitis ELISA kit
BioLisa® kit Bovine Digital Dermatitis Ab
The Digital Dermatitis ELISA kit is an indirect single-step ELISA test for the detection of antibodies to Treponema phagedenis in bovine tank milk samples. This ELISA test allows the epidemiological monitoring of Digital Dermatitis or Mortellaro's disease.
✔ Digital Dermatitis ELISA kit developed following French standards U47-310 and U47-019 (AFNOR)
✔ Ready-to-use and colored reagents for fast, easy differentiation
✔ Indirect single-well ELISA test for Digital Dermatitis (in breakable strips), sensitive and specific, allowing the detection of a bovine herd's exposure to Treponema phagedenis
✔ Short incubation protocol
✔ To improve the monitoring of your results over time, we suggest the use of a ready-to-use low-positive sample (MRI, Matériel de Référence Interne, BioLisa® MRI Milk Bovine Digital Dermatitis (low prevalence), et BioLisa® MRI Milk Bovine Digital Dermatitis (moderate prevalence), ref. ELTBDD001 et ELTBDD002). They allow you to monitor the reliability of the test using a control card. We recommend using them with each test.
✔ Matrices: Sample of bovine tank milk (milk from large mixtures)
✔ Used for the epidemiological monitoring of Digital Dermatitis in dairy herds, this test makes it possible to monitor and track the disease's prevalence and thus adapt sanitary measures for its management.
✔ Made in France
- Method
- Indirect ELISA
- Species
- Ruminants
- Pathogen
- Treponema phagedenis
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
About Digital Dermatitis (Mortellaro's disease)
Digital Dermatitis, also known as interdigital dermatitis, is an infectious foot disease of cattle, caused mainly by bacteria of the genus Treponema. These bacteria, notably Treponema medium and Treponema phagedenis, are the main pathogens associated with this disease.
➜ Symptoms: Digital Dermatitis is characterized by inflammatory lesions between the hooves of cattle. These lesions may include redness, swelling, ulceration, purulent discharge, and occasionally areas of tissue necrosis. Affected cattle may limp and show signs of pain and discomfort when walking.
➜ Propagation: The disease generally spreads in environments where hygiene conditions are inadequate. Bacteria of the Treponema genus are often present in soil and mud, and infection can occur when cattle come into contact with contaminated surfaces.
➜ Risk factors: several factors can contribute to the development of Digital Dermatitis in cattle, including damp and dirty environmental conditions, the presence of wounds or abrasions in the feet, stress, nutritional deficiencies, and the presence of other pathogens in the environment.
Diagnosis & prevention of Digital Dermatitis (Mortellaro's disease)
Treatment is mainly based on the local application of antibiotics (such as oxytetracycline spray) following hygienic hoof trimming to clean the infected area. In the most severe cases, temporarily isolating the affected animal may be necessary.
Prevention is essential and relies on strict hygiene in housing facilities (maintaining clean and dry floors), the regular use of disinfectant footbaths (e.g., copper sulfate, formalin), routine preventive hoof trimming, and close monitoring of the herd.
Although Mortellaro’s disease is highly contagious, it is not transmissible to humans. It is a multifactorial disease that requires a comprehensive management approach combining hygiene, prevention, care, and health monitoring.
BioSellal has developed an innovative ELISA kit for the detection and monitoring of antibody levels in bulk tank milk: the BioLisa® kit Bovine Digital Dermatitis Ab (for bulk tank milk), as well as in individual serum using the BioLisa® kit Treponema phagedenis Ab, as part of the surveillance of infectious diseases in cattle farming.
The use of such tests on both bulk tank milk and individual serum is particularly relevant for large-scale surveillance programs, as it allows the monitoring of immune status without the need to individually sample every animal in the herd.
Made in France
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 24 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning delivery, lead times, expiry dates, batches, etc.)
Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all questions regarding information on a product, a quote, a partnership, etc.)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance on our products or for all questions following analyses)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
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