H5 and H7 Avian Influenza PCR Kit
Bio-T kit® AIV genotypes H5 & H7 V2
The H5 & H7 Avian Influenza PCR Kit is a ready-to-use kit for the detection of Avian Influenza A and H5 & H7 (H5 & H7) virus genomes by real-time PCR, for the diagnosis of Avian Influenza. This Avian Influenza H5 and H7 PCR test contains an endogenous IPC internal positive control and an exogenous IC internal control. It has been validated by the French National Reference Laboratory for Avian Influenza (ANSES, Ploufragan-Plouzané-Niort).
✔ Avian Influenza H5 and H7 quadruplex PCR kit developed following French standard U47-600-2 (AFNOR) and validated by the French Avian Influenza LNR (ANSES, Ploufragan-Plouzané-Niort)
✔ Rapid thawing of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Reliable detection of Avian Influenza H5 and H7 by real-time PCR thanks to 2 IPCs: an exogenous IPC and an endogenous IPC to ensure high-quality results on all matrices, even the most difficult ones
✔ Endogenous IPC detection verified on a wide panel of avian species including Hen, Duck, Turkey, Peregrine Falcon, Tawny Owl, Warbler, Buzzard, Wood Pigeon, Turtle Dove, Grosbeak, Robin, Stork, Buzzard...
✔ Sensitivity & specificity of Avian Influenza diagnosis with our extraction kits: 100% on organs or organ shreds (intestine, brain), swabs, and environmental samples such as wipes and boot cloths.
✔ Matrices: tracheal or oropharyngeal swab, cloacal swab, organ or organ shred supernatant, rag or boot cloths. Individual analyses or mixtures of up to 5 depending on the sample type and, unless otherwise specified, on the animal species, geographical origin, and date of sampling.
✔ Kit developed on the AriaMx™ (Agilent Technologies, Fast ramping by default) and confirmed on ABI PRISM® 7500 Fast (Applied Biosystems) in standard ramping, QuantStudio™ 5 (Applied Biosystems) in standard ramping, CFX96™ (Bio-Rad) in standard ramping and Mic qPCR Cycler (Bio Molecular Systems) in standard ramping. It is compatible with all thermal cyclers with at least 6-FAM, VIC, Cy5, and Texas Red reading channels. For further information, please contact our technical support.
✔ Kit validated on Mic qPCR cycler (Bio molecular systems) with 60 min run
✔ Made in France
- Method
- qRT-PCR
- Species
- Avian
- Pathogen
- Avian influenza virus
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
About H5 and H7 avian influenza
Avian Influenza, caused by specific subtypes of the Avian Influenza virus such as H5 and H7, is a serious viral disease that mainly affects birds. The H5 and H7 avian influenza viruses are of particular concern because of their high potential for spread and their ability to cause severe epidemics in avian populations.
Symptoms of H5 and H7 avian influenza in birds include reduced egg production, respiratory signs such as coughing and sneezing, swelling of the head and face, and neurological signs such as paralysis.
Diagnosis & prevention of H5 and H7 avian influenza
In addition to the significant economic damage it can cause to the poultry industry, Avian Influenza also represents a potential threat to public health due to its ability to spread from animals to humans. This is why early and accurate diagnosis of H5 and H7 Avian Influenza is essential to control its spread and take appropriate measures. H5 H7 Avian Influenza PCR kits, such as those developed by BioSellal, offer an effective solution for detecting the presence of H5 and H7 avian influenza virus subtypes in avian samples, enabling farmers, veterinarians and health authorities to intervene rapidly to limit the spread of the disease and protect the health of avian and human populations.
Made in France
Opt for quality and local know-how.
Iso-certified
A guarantee of quality in line with international standards.
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.