Lumpy skin disease PCR kit DIVA V2
The Lumpy Skin Disease DIVA PCR kit is a ready-to-use kit for the differential detection (DIVA) of wild and vaccine Neething strains of Lumpy Skin Disease (LSD) virus by real-time PCR, for the diagnosis of Contagious Nodular Dermatosis (CND) in cattle. This Lumpy Skin Disease PCR test contains an endogenous IPC internal positive control.
✔ Lumpy Skin Disease PCR Kit DIVA V2 triplex developed in compliance with French standard U47-600-2 (AFNOR)
✔ Rapid thawing of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Clear, reliable differentiation between naturally infected animals (clade 1.2) and those vaccinated (clade 1.1 - Neethling vaccine)
✔ Optimized detection of recombinant strains (clade 2.5) as wild strains, guaranteeing classification in line with epidemiological reality.
✔ Equivalent sensitivity between the two detection systems, vaccine (FAM) and wild (VIC), ensuring reliable and comparable results on all types of samples.
✔ Simplified, secure kit use thanks to easy reading of results and integrated endogenous control guaranteeing good sample quality and absence of inhibition.
✔ Matrices: Whole blood (on EDTA tube), oral, nasal, conjunctival dry swab, skin lesion swab, skin lesion scraping and nodule biopsy. Individual analyses.
✔ Compatible with all thermal cyclers with at least reading channels 6-FAM, VIC and Cy5. For further information, please contact our technical support.
✔ Extraction and amplification program common to all Ruminant species kits
✔ Made in France
In the context of official analysis, it is necessary to comply with the recommendations of local authorities and/or the Laboratoire Nationale de Référence. In France, in the event of clinical suspicion, only the sampling and testing scheme defined by the DGAI and the LNR, as described in the "Guide pratique de diagnostic et de gestion des épizooties : DNC", drafted in 2010 and updated 2025, must be followed.
- Method
- qPCR
- Species
- Ruminants
- Pathogen
- Lumpy skin disease virus
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
Made in France
Opt for quality and local know-how.
Iso-certified
A guarantee of quality in line with international standards.
Secure payment
Your transactions are completely secure.
Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 24 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning delivery, lead times, expiry dates, batches, etc.)
Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all questions regarding information on a product, a quote, a partnership, etc.)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance on our products or for all questions following analyses)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.