Lumpy skin disease PCR kit
Bio-T kit® Lumpy Skin Disease
The Lumpy Skin Disease PCR kit is a ready-to-use kit for the detection of LSD (Lumpy Skin Disease) virus by real-time PCR, for the diagnosis of Lumpy Skin Disease in cattle. This Lumpy Skin Disease PCR test contains an endogenous IPC internal positive control. It has been evaluated by the Pirbright Institute, UK.
✔ Lumpy Skin Disease duplex PCR kit developed following French standard U47-600-2 (AFNOR) and evaluated by the Pirbright Institute (UK)
✔ Rapid thawing of the ready-to-use, blue-colored Master Mix for easy distinction
✔ Reliable detection of LSD by real-time PCR using endogenous IPC to ensure high-quality results on all matrices, even the most challenging ones
✔ Matrices: whole blood (on EDTA tubes), dry oral, nasal, conjunctival swabs, and skin lesion scrapings. Individual analyses.
✔ Kit developed on ABI PRISM® 7500 Fast (Applied Biosystems) in standard ramping and confirmed on ABI PRISM® 7500 Fast (Applied Biosystems) in Fast ramping and AriaMx™ (Agilent Technologies, Fast ramping by default). It is compatible with all thermal cyclers with at least 6-FAM and Cy5 reading channels. For more information, contact our technical support.
✔ Extraction and amplification program common to all Ruminant species kits
✔ Made in France
- Method
- qPCR
- Species
- Ruminants
- Pathogen
- Lumpy skin disease virus
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
Contagious nodular dermatitis in cattle
Contagious Nodular Bovine Dermatosis, more commonly known as Lumpy Skin Disease (LSD), is a highly contagious viral disease that mainly affects cattle. The disease is caused by a poxvirus of the Capripoxvirus type. Symptoms of LSD include the appearance of cutaneous nodules on various parts of the bovine body, notably the skin, mucous membranes and genitals. These nodules can cause itching and pain in infected animals, leading to loss of appetite, reduced milk production and respiratory difficulties in severe cases.
Diagnosis & prevention of Contagious Nodular Dermatitis in cattle
The disease can be spread by direct contact between infected animals, or by insect vectors such as flies. Early diagnosis of Lumpy Skin Disease is essential to implement appropriate control measures and limit its spread within herds. Lumpy Skin Disease PCR kits like the one developed by BioSellal offer a rapid and accurate solution for the detection of LSD in animal samples, enabling farmers and veterinarians to take proactive measures quickly to limit the spread of the disease and protect the health of their herds.
Vaccination against Lumpy Skin Disease (LSD) involves the use of live attenuated vaccines. These vaccines contain a weakened form of the Lumpy Skin Disease virus, which stimulates an immune response in cattle without causing disease. A commonly used vaccine contains the Neethling strain of LSD virus, which has been attenuated to reduce its virulence while still inducing effective protection. Vaccination is administered subcutaneously or intramuscularly, and plays a crucial role in preventing the spread of the disease and reducing associated economic losses.
Made in France
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.