Dermatitis digititis ELISA kit
BioLisa® kit Bovine Digital Dermatitis Ab
The Digitised Dermatitis ELISA kit is an indirect single-step ELISA test for the detection of antibodies to Treponema phagedenis in bovine tank milk samples. This ELISA test is used to diagnose Digitis Dermatitis or Mortellaro's disease.
✔ Kit ELISA Dermatite digitée developed following French standards U47-310 and U47-019 (AFNOR)
✔ Ready-to-use, color-coded reagents for fast, easy differentiation
✔ Sensitive and specific ELISA test for Treponema phagedenis exposure in cattle
✔ Short incubation protocol possible
✔ To improve the monitoring of our results over time, we suggest the use of a ready-to-use low-positive sample (MRI, Matériel de Référence Interne). This makes it possible to monitor the reliability of the test using a control graph. It is recommended to use it for each test.
✔ Matrices: Sample of tank milk (milk from large mixtures) in cattle
✔ Used to diagnose Digitis Dermatitis in dairy herds, this test makes it possible to monitor and track the disease's prevalence and thus adapt sanitary measures for its management.
✔ Made in France
- Method
- Indirect ELISA
- Species
- Ruminants
- Pathogen
- Treponema phagedenis
- Brand
- Biosellal
Resources
Download our marketing documents below for more information.
About digitis dermatitis (Mortellaro's disease)
Dermatitis digitata, also known as interdigital dermatitis, is an infectious foot disease of cattle, caused mainly by bacteria of the genus Treponema. These bacteria, notably Treponema medium and Treponema phagedenis, are the main pathogens associated with this disease.
➜ Symptoms: digitis dermatitis is characterized by inflammatory lesions between the hooves of cattle. These lesions may include redness, swelling, ulceration, purulent discharge, and occasionally areas of tissue necrosis. Affected cattle may limp and show signs of pain and discomfort when walking.
➜ Propagation: the disease generally spreads in environments where hygiene conditions are inadequate. Bacteria of the Treponema genus are often present in soil and mud, and infection can occur when cattle come into contact with contaminated surfaces.
➜ Risk factors: several factors can contribute to the development of digitis dermatitis in cattle, including damp and dirty environmental conditions, the presence of wounds or abrasions in the feet, stress, nutritional deficiencies, and the presence of other pathogens in the environment.
Diagnosis & prevention of Dermatitis digitorum
Diagnosis of digitis dermatitis is usually based on clinical observation of characteristic lesions between the hooves of cattle. In some cases, tissue samples may be taken for microbiological analysis to specifically identify bacteria of the Treponema genus. Serological analysis of tannin milk can correlate with the prevalence of this disease in a dairy herd. The ELISA kit for Treponema dermatitis developed by BioSellal offers a reliable and accurate solution for diagnosing this disease, enabling veterinarians and breeders to take proactive measures to control it and preserve herd health, as well as monitoring the effectiveness of these measures over time.
Made in France
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.