E. coli Bovine Serotyping PCR Kit
Bio-T kit® E. coli Bovine Serotyping
The E. coli Bovine Serotyping PCR kit is a ready-to-use kit for the detection of the adhesion factors F17A, Fim41, CS31A and F5 in Escherichia coli (E. coli).
✔ E. coli Bovine Serotyping quintuplex PCR kit
✔ Rapid thawing of the ready-to-use, blue-coloured Master Mix for easy distinction
✔ Reliable detection of the adhesion factors F17A, Fim41, CS31A and F5 in Escherichia coli (E. coli):
- Adhesion factor F17A (F17A), with 6-FAM labelling,
- Adhesion factor Fim41 (Fim41), with VIC labelling,
- Adhesion factor CS31A (CS31A), with Texas Red labelling,
- Adhesion factor F5 (F5), with Cy5 labelling,
- A positive control (E. coli) targeting the ybbW gene, with ATTO700 labelling, which confirms the presence of Escherichia coli as opposed to other bacterial or enteric species.
✔ Matrices: bacterial colonies, faeces. Individual analyses.
✔ Kit developed on CFX96™ (Bio-Rad) and confirmed on QuantStudio™ 5 Real-time PCR System (Applied Biosystems™), Mic qPCR Cycler (Bio Molecular Systems) and AriaMx™ Real-Time PCR System (Agilent Technologies). For more information on thermal cycler compatibility, contact our technical support.
- Method
- qPCR
- Species
- Ruminants
- Pathogen
- E. coli (F17A/Fim41/CS31A/F5)
- Brand
- Biosellal
Made in France
Opt for quality and local know-how.
Iso-certified
A guarantee of quality in line with international standards.
Secure payment
Your transactions are completely secure.
Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 24 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning delivery, lead times, expiry dates, batches, etc.)
Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all questions regarding information on a product, a quote, a partnership, etc.)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance on our products or for all questions following analyses)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.