BTV PCR Kit
Bio-T kit® BTV all genotypes
The BTV PCR kit is a ready-to-use kit for the detection of all strains of Bluetongue virus (BTV) by real-time PCR for the diagnosis of Bluetongue. This BTV test contains an endogenous IPC internal positive control. It is validated by the French FCO National Reference Laboratory (ANSES, Maisons-Alfort).
✔ BTV duplex PCR kit developed following French standard U47-600-2 (AFNOR) and validated by the French FCO LNR (ANSES, Maisons-Alfort)
✔ Rapid defrosting of ready-to-use, blue Master Mix for easy distinction
✔ Reliable detection of BTV by real-time PCR using endogenous IPC to ensure high-quality results on all matrices, even the most difficult
✔ Matrices: Whole blood (on EDTA tubes). Individual analyses.
✔ Kit developed on ABI PRISM® 7500 Fast (Applied Biosystems) in standard ramping and confirmed on AriaMx™ (Agilent Technologies, default Fast ramping) and ABI PRISM® 7500 Fast (Applied Biosystems) in Fast ramping. For other thermal cyclers, don't hesitate to contact our technical department.
✔ Kit validated on the Mic qPCR cycler (Bio molecular systems) with 60 min run
✔ Extraction and amplification program common to all kits for Ruminant species
✔ French manufacture
- Method
- qRT-PCR
- Species
- Ruminants
- Pathogen
- Bluetongue virus
- Brand
- Biosellal
Resources
Download our marketing documents for more information.
About Bluetongue
Bluetongue (BTV) is a viral disease primarily affecting sheep and cattle. Caused by the BTV virus, the disease is transmitted by biting insects such as Culicoides midges. Clinical signs of FCO include fever, mucous membrane congestion, excessive salivation, swelling of the tongue and lips, mouth ulcers, respiratory difficulty, locomotor disorders, and sometimes death in severely affected animals.
Diagnosis & prevention of bluetongue
DIAGNOSTICS
➜ Diagnostic tests: The diagnosis of FCO relies on laboratory tests, including serological tests such as ELISA (Enzyme-Linked Immunosorbent Assay) to detect the presence of antibodies directed against the virus, as well as molecular tests such as PCR (Polymerase Chain Reaction) to detect viral RNA in blood or tissue samples.
➜ Vector identification: Monitoring populations of Culicoides, the insect vectors, is also important for assessing the risk of disease transmission in a given region.
PREVENTION
➜ Vaccination: Vaccinating animals is one of the most effective ways of preventing FCO. Inactivated vaccines are available to protect sheep and cattle against different serotypes of the FCO virus.
➜ Vector control: Management of vector midge populations is essential to reduce the risk of transmission of FMD. This can include the use of insecticides, insect habitat management, and other environmental control measures.
➜ Animal Movement Control: Limiting animal movements from infected to uninfected areas can help prevent the spread of FCO.
➜ Surveillance and Notification: Continuous disease surveillance, including notification of suspected cases, is essential for early detection of outbreaks and implementation of appropriate control measures.
In summary, early diagnosis, vaccination, vector control and animal movement management are key elements in the prevention and control of Bluetongue. An integrated, proactive approach is needed to limit the impact of this viral disease on sheep and cattle herds.
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A guarantee of quality in line with international standards.
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Availability & support
Our technical support team is on hand to help you with your analyses.
Frequently asked questions
Each real-time PCR kit includes :
- Ready-to-use Master Mix (blue color)
- Positive amplification control, containing targeted pathogens - EPC
- Negative amplification control - water.
- Exogenous IPC (Internal positive control)
Each ELISA kit includes at least :
- Test plate with 12 x 8-well strips
- Sample diluent
- Negative control
- Positive control
- 100X or ready-to-use conjugate
- Conjugate diluent
- Wash solution
- Substrate
- Stop solution
- Plate adhesives
Each product comes with a certificate of analysis and detailed instructions for use to ensure optimum use and reliable results.
To ensure the integrity and efficiency of your kits, follow these storage recommendations:
Real-time PCR kits:
On receipt: Store kits at a temperature ≤ -16°C. This preserves the quality and performance of the kit components.
ELISA kits:
On receipt: Store components at 5 ± 3°C to maintain efficacy. The concentrated wash solution can be stored at room temperature, i.e. 21 ± 4°C.
By respecting these storage conditions, you ensure the reliability and optimum performance of your kits for accurate and consistent results.
- The shelf life of a PCR kit for the detection of RNA pathogens (viruses) is 12 months.
- The shelf-life of a PCR kit for the detection of DNA pathogens (viruses, bacteria, parasites) is 18 months.
- The shelf-life of an ELISA kit is 15 or 18 months.
At BioSellal, we are constantly seeking to improve our products. We regularly carry out stability studies to extend expiration dates, guaranteeing that our kits will always perform better and last longer.
Yes, absolutely! At BioSellal, we're committed to providing our customers with complete solutions to carry out their analyses under optimum conditions. That's why, for the majority of our PCR and ELISA kits, we offer in-house reference materials, guaranteeing you reliable, accurate results every time.
The quality management system implemented by BioSellal was certified ISO 9001 (version 2015) by AFNOR in June 2017 and has since been renewed for the following areas: Research and development activities, production, industrialization and sales and technical support of PCR and ELISA kits for veterinary diagnostics. BioSellal carries out several levels of quality control on product batches, as well as validation of raw materials.
All PCR kits are developed and validated to NF U47 600-2 standard. All ELISA kits are developed and validated in accordance with standards NF U47-310, NF U47-301, NF U47-300, NF U47-020, NF U47-019.
Yes, that's right! Our PCR kits are designed to be compatible with all open devices with FAM, VIC/HEX, CY5 and Texas Red/ROX reading channels. So you can use them with confidence on a wide range of thermal cyclers for optimum results.
At BioSellal, we have a particular preference for the MIC thermal cycler, the most compact, accurate and fast thermal cycler on the market.
Our various departments are always ready to answer your questions and help you. Here's how to contact us:
Sales Administration & Logistics (for all questions concerning lead times, expiry dates, batches, etc.)
- Mail: contact@biosellal.com
- Phone: +33 (0)4 26 78 32 48
Sales Department (for all customer inquiries)
- Mail: commercial@biosellal.com
- Phone: +33 (0)4 26 78 32 57
Technical Support (for technical assistance)
- Mail: tech@biosellal.com
- Tel: +33 (0)4 26 78 32 48
Our dedicated teams are here to ensure you have the best possible experience with our products. We look forward to hearing from you!
Absolutely! At BioSellal, we offer customized training to maximize the use of our technologies, including real-time PCR, ELISA, sequencing and digital PCR. Whether you want to discover our products or deepen your knowledge, we're here to support you every step of the way.
All documents relating to our products are available directly on our website. Simply create an account and log in for easy access to all our documentation resources.